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"GT" Pesticide test-kit

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Question about GT-test kit


    Question about GT test kit/ Question 1

Test tubes with micro-biological vegetables like garlic and onion for example gives a equally dark/or darker color than the comparison test tube, even though the farmer insisted that no insecticides were used, and only naturally extracted chemicals were used.

 

    Answer

Samples of micro-biological vegetables are commonly sprayed by Neem tree-extracted chemical or EM for example. If used with a suitable amount as substitute for the use of pesticides, it should give a lighter or as dark color as the reference test tube. However, if the amount of these chemical used is over a certain limit, then the results given by the GT test kit will always be darker than the reference test tube. The intensity of the color shows the amounts of toxicity that varies according to many factors such as the different cell tissues in the vegetables or different types of fruits and vegetables may neutralizes the toxin or even enhance the toxin. Even if the vegetables are of the same species but grown in different conditions such as good rain or dried land can affect the results. For example, recently a toxin-free onion was tested once but gives the same color as the reference tube. Afterwards, it was found that the onion from the same source gives a darker color than the reference test tube. This proves that other factors like dryness or excessive use of naturally-extracted chemicals will make the resulting color darker. Nevertheless, if the result is darker than the reference test tube, then it is unsafe. What actually happened is that the toxin (that is not an insecticide) that the ezymecholinesterase tested for the consumers, while the standard method cannot determine a result. Therefore farmers must consider the amount of organic chemicals carefully before using them.


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    Question about GT test kit/ Question 2

Tested a sample of pepper that reportedly did not contain any insecticide and found it to be unsafe (darker than or as dark as the reference test tube). Sample may or may not be rejected.

 

    Answer

From the study made on a 5g sample of pepper, we found that each species of pepper contains inhibit chemicals with different enzymes. This is probably due to the varying spiciness of each pepper, thus making the color as dark as the controlled tube, and some can even be slightly darker than the controlled tube up to almost as darks at the deciding-tube.

Consequently, in the meeting of the auditors from the Toxic-residue testing support project decided that they will allow the testing standards to use at 4 times less pepper; meaning 2.5g of sample per 10ml of GT-1.
This is to discard the possibility that the toxin in the sample might interfere with the test. When the amount of sample is reduced the sample test tube is darker or as dark as the deciding test tube, therefore it cannot be excused that it came from the sample. Another reason that the committee decided to lower the amount of pepper sample used is the fact that pepper is consumed in a far smaller amount than other vegetables anyway. In addition, the standard test will also require the sample to be at least 2-5 times more diluted before injecting it into the Gas Chromatography.


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    Question about GT test kit/ Question 3

A sample of vegetable was tested unsafe by the GT test kit, but the standard lab test found it safe.

 

    Answer

The standard test in the lab uses the GC and HPLC machine to filter out samples; this is different to the way the GT test kit works. The standard test will use a method called Multiresidues Method, which tests 4 groups of chemicals: Chlorine, phosphate, carbamate and pyritroid. To determine which toxin is in the sample, a standard sample must be used to compare. If the toxin has no comparison sample or if the toxin changed form/decomposed into other toxins or the toxin created didn’t come from the insecticide; the standard method will not work. However, the GT test kit tests the toxicity of the toxic residue in the vegetables, therefore no matter what happens to the chemicals in the sample, the GT test kit can detect it. Therefore it is possible that the GT test kit may find a sample to be unsafe, but the standard test might not find anything. In conclusion, the following are the reasons:
-a chemical in one of the 4 mentioned groups or another group that cannot be analyzed
-a chemical or many chemicals in one of the 4 mentioned groups but in such a small quantity that the equipment cannot test for it. But even if the quantity is small, it can still react with the cell tissue of the vegetables and cause high toxicity.


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    Question about GT test kit/ Question 4

Vegetable grown using microbiological-farming style using Neem tree-extracted chemical spray. When harvested, the buy used GT test kit and found that most of the produce gives a slightly darker or as darks as the reference test tube, except the onion which gives a matching color and darkness.

 

    Answer

Just like question 1, the amount of naturally extracted chemical can still cause toxicity if overused.


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    Question about GT test kit/ Question 5

Used the GT test kit to test consumption-ready food, how should the result be interpreted?

 

    Answer

Consumption food means food that have been processed by cleaning, well done or raw, such as vegetable salad. Each process can eliminate all toxins if the toxic residue is within the safety limits for consumers. Therefore consumer-ready food, if tested with the GT kit, should not find any toxin in the food and the color in the sample test tube must not be darker than the controlled test tube.


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    Question about GT test kit/ Question 6

Is the GT test kit capable of testing water and soil? And how should the results be interpreted?

 

    Answer

The GT test kit can test for toxic residue in samples of water/soil by extracting the sample as mentioned in the test kit manual. For interpreting the result, if the sample is water/soil from filthy sources or water/soil from the garden, the test can tell the level of toxicity (unrelated to safety/unsafe). If the purpose of the test is to test drinking water then the test should not find any toxic residue, meaning the color should not be darker than the controlled test tube.


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    Question about GT test kit/ Question 7

Drinking water might be contaminated; will the GT test kit work?

 

    Answer

If the drinking water is contaminated with neurotoxins then the GT test kit will be able to detect the toxin.


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    Question about GT test kit/ Question 8

GT test kit with 10 tests and 30 tests respectively did not test properly.

 

    Answer

The GT test kit requires careful planning, if the type of kit is 10 tests and 10 tests must be made, then 10 extracted samples + controlled test tube + deciding test tube a total of 12 tests. The same idea applies to the 30 tests type.


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    Question about GT test kit/ Question 9

Why the modified tray in GT’s equipment has been opened for hour but the temperature was not at specific point 35 celsius?

 

    Answer

When temperature is not at the specific point (+or - 2 from 35 celsius) within hour, you have to look at your work environment that you put the tray near by air condition or near fan. The solution is make sure that the air from fan or air condition blow not directly to your experiment; otherwise, put the warm water into the tray instead of tap water especially when the winter season. We comment you to put the warm water into the tray as the temperature can get to the specific point faster than using tap water.

7/ Nov/ 04


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   Question about GT test kit/ Question 10

Why the light in modified stove open does for hour, the water was not boiling?

 

    Answer

You have to recheck your understanding again because the temperature that use in GT’s experiment is human’s temperature or about 37 celsius. The temperature of the testing therefore is about +or - 2 from 35 celsius.

7/ Nov/ 04


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    Question about GT test kit/ Question 11

What is organic agriculture?

 

    Answer

Organic agriculture is best known as a method of agriculture where no synthetic fertilizers and pesticides are used. This description does not mention the essence of this form of agriculture, however, which is the management of farms in such a way that soil fertility and pest problems are prevented. Although many single techniques used in organic agriculture are used in a wide range of agricultural management systems, what differentiates organic agriculture is the focus of the management. Under the organic system, the focus is on maintaining and improving the overall health of the individual farm's soil-microbe-plant-animal system (a holistic approach), which affects present and future yields. The emphasis in organic agriculture is on using inputs (including knowledge) in a way which encourages the biological processes of available nutrients and defenses against pests, i.e., the resource "nature" is manipulated to encourage processes which help to raise and maintain farm productivity. The soil is a central part of that system. Most fertilizers and pesticides are considered to hinder that process and are, therefore, prohibited. In organic agriculture, management is directed towards preventing problems, while stimulating processes which assist in nutrition and pest management.
For market purposes, a strict definition of organic agriculture is required to protect both producer and consumer interests. Definitions were first developed in the private sector. The most widely adopted definition was developed and promoted by the International Federal of Organic Agriculture Movements (IFOAM), a non-governmental organization that has existed for 25 years. The IFOAM Principle Aims are used as guidelines for setting standards for organic agriculture in individual countries.
You can read more on FAO

13/ Jan/ 05


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    Question about GT test kit/ Question 12

Is organic agriculture necessarily checked for pesticide residue?

 

    Answer

It is necessary to be checked with GT-test kit. The reason is that even the pesticides or fertilizer is prohibited in organic agriculture; herb extracted has been used instead. The result of using herb extract, which aim on protecting plant from insect the same way as pesticide and fertilizer do, still have affect that would leave to consumers. Think of the herb extract can harm insect, so it would also harm us. Some formulas use hazard herbs, which human can be dead by having them, for example, หางไหลแดง, หางไหลขาว, มะกล่ำ. Furthermore, because of unawareness of using them, there is no research taking seriously. GT-test kit can test toxicant from herb extract since enzyme in GT-1 can detect pesticide residues. Meanwhile, there are no test kits for checking residues in herb extract even standard method.

13/ Jan/ 05


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   Question about GT test kit/ Question 13

Why the result of pesticide residue testing in organic vegetable still had the pesticide residue even it was not used with any pesticides? and what is suitable standard control for organic vegetable?

 

    Answer

From the result of GT-pesticide test kit, there were residue leave in organic vegetable because it was used herb extract for protecting plants the same as pesticide do. The reason is that the chemical from herb extract still have toxicant, which inhibit enzyme. The producers and formers therefore should have instruction of using the herb extract for example the consistency of using and appropriate date of stopping before harvest and deliver to consumers. In addition, the limited control of GT-method for organic agriculture is about 0-10%(zero to ten percent) this mean judge tube(หลอดตัดสิน) have to be 10 % inhibition so the color of sample tube should be ? 10%(less or equal ten percent).

13/ Jan/ 05


 

Note: these are frequently asked questions, therefore we didn’t include any names and company for credit.

 

 

 

 

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