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GT test kit Developer

 

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Pesticides, better known as insecticides, are poisonous substances used to prevent and terminate pests of agricultural products like vegetables, fruits, grains for example.

However the amount of toxic in these foods must be at a safe level, which brings us to the fact that testing for the toxicity of these foods can be very complicated. Expensive equipments are required, the tester has to be very skillful and experienced, accompanied by the fact that the test takes a long time to complete, which turns out to be too slow considering the fast deterioration rate of agricultural products. The safety level must also be compared to international standards as well. However, comparison often brings complications since the chemicals found in certain samples don’t have a specified value, therefore it is difficult to determine if the toxin found in the food is safe or not.

Therefore the invention and development of semi-standard solvent test kit is useful in filtering out unsafe samples. The safety levels are made from the toxicity of one toxin or the combined toxicity that hampers the performance of the Enzyme-chlorine-esterase by at least 50%. This safety level is then compared and analyzed with results from standard lab results. We found that the result is satisfactory and the results were very reasonable. For that reason this test kit is suitable for filtering out unsafe food for the consumers in time for their needs. This test kit will only take 60 minutes to run, and is quite accurate; ideal for testing and controlling the cleanliness of fresh fruits and vegetables before selling. It can also be used for research that involves testing many samples in a series or to expand the investigation of toxicity in soil and water. For users who need speed, we have developed the test kit and reduced the testing time from 60 minutes to 15 minutes and 30 minutes respectively, this gives the customers a variety of choice.

Many theorists have questioned the testing method of fruits and vegetables using cholinesterase inhibition technique. This technique can only test 2 groups of toxins: Phosphates and Carbamates. Other groups like Chlorinepyritroid, fungicide for example. Therefore it doesn’t mean that if the tester tested negative, there won’t be any toxins in the vegetables. Since, as I have to admit “There is yet to be any testing method that is capable of detecting every single kind of toxin in vegetables, even testing methods made in the lab using expensive equipments still needs the test to be divided into groups, each group based on the ability of the tester, which varies greatly. Therefore is the standard tested negative, it doesn’t mean that there aren’t any toxins in the sample, especially since the result of the test greatly depends on the ability of the tester and the chemical used to test. In the case that the toxin in the sample changes or decomposes after reacting with the plant tissue and forms a new chemical that is even more toxic, this cannot be detected using the standard testing method. However the Cholinesterase inhabitation technique uses enzyme to determine toxicity, so the enzyme is able to easily and clearly detect the toxicity.
In the other case that chemicals extracted from the nature is used to neutralize insecticides is used on Micro-agricultural products, a frequent dose of it can lead to accumulation and toxicity. This often happens when users think the product is “natural” and overuses it. In conclusion, this skim-test is extremely useful in filtering out unsafe products and is inexpensive and takes up only a short period of time such that the food won’t decay. This is one of the best methods around until new methods are invented.


Qualification


Standard testing
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GT-test kit testing

Investment

High

Low

Time

sending experiment(30 days)

Depending on GT-1
(30,60 mins)

Quantity use of chemical

High

Low

Tester experience

Required

Not required

Waste

Have a large quantity and need to be eliminated by technicain

Have a small quantity
and can be eliminated
by non technician

Reading Result

1. Difficult to read the result as detected toxic is depending on ability of tester and standand chemical to compare sorely. Therefore, in case of non experience tester and no chemical comparison would be unable to detect the poisonous.

1. Easy to read the
result by comparing
the color of control
and critical tubes
(Detail in " Introduction
to basic testing
")Since,
tester can sightly
compare.

2. When the toxic is conterminated with other(s), it changed after used/dissolved or reactioned with cell plant; the new toxic, which has more toxicity, cannot be tested.

2. The result cannot
be addressed whether
it is in which type or
group; however, the
color itself can notified
the toxic that has been
detected is in the safe
level or not.

3. When the poisonous has been addressed which type or group it is if there is no MRL index within Thai and others, tester will not be able to specify that it is safe to be consumed or not.

3. In case of contamination/dissolve/
reaction with cell plant,
the enzyme in GT-test
kitwill show the
toxicity obviously.


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