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(1) Pour 5g (fill up to level 2 on the plastic bottle) of finely cut food sample into the plastic bottle.

(2) Pour 5cc or enough to cover the sample, of extracted solvent, shake well and leave it for 10-15 minutes.

(3) Suck out 1cc of the solvent from instruction no. 2 into a test tube and then place the tube into an evaporation basin. Heat until all the solvent has evaporated.

From now on all methods will be operated in water bath (as in picture 3)

(4) Label and fill in 3 new test tubes with the following:

(4.1) Test tube 1 labels "Judge" tube: 1cc of solvent 2

(4.2) Test tube 2 labels "Control"tube: 1cc of solvent 2

(4.3) Test tube 3 label "Sample" tube: 1cc of sample extract from instruction no.3.

(5) Add 0.50cc of GT-1 into each test tubes, leave for 5-10 minutes.

(6) While waiting as specified in instruction no.4, pour GT-2.1 into GT-2 to make a “mixed solvent GT-2” and then pour GT-3.1 into GT-3 to make “mixed solvent GT-3”.

(7) Pour 0.375cc of “mixed solvent GT-2” into test tube 1, pour 0.25cc of “mixed solvent GT-2” into test tube 2 and 3 and leave them for 30 minutes.
(8) Pour 1cc of “mixed solvent GT-3” into each test tubes followed by 0.5cc of GT-4. Shake well and then add 0.5cc of GT-5, shake well, compare and analyze the colors with the reference table.

Reading result table

Color in sample tube(s)

Result

Sample tube has less color than control tube.

1. Not Detect

Sample tube has greater color than control tube.

2. Detect but safe to be consumed

Sample tube has greater color than critical tube.

3. Detect and not safe